Yeast Cell Disruption
Note: With yeasts cells that require higher pressures there is often a balance of whether to go to with higher pressure for more cell lysis in a single pass or to multi pass at lower pressure. Higher pressure results in higher temperature which may, in some cases, be detrimental to protein recovery (which can be counteracted by our cooling systems). We can help and advise on HPH processing parameters for your application.
The before and after processing images below demonstrate the high lysis efficiency our STANSTED homogenizers can achieve.
Fig A, B - Unprocessed Yeast suspension.
Fig C, D, E - Processed Yeast suspension, after Cell Disruption, using different processing conditions.
Fig A
Fig B
Fig C
Fig D
Fig E
The STANSTED high pressure homogenizer range provides optimal combination of processing pressure and passes for all types of yeast cells with repeatable results.
Yeast Cells - Benefits of our Technology
Process pressures from 1,500 psi (100 bar) to 60,000 psi (4200 bar) - the highest available for this technology.
Multiple options for temperature control incuding pre-heating and rapid post process cooling.
Process volumes from 1m to 275ml per minute in the Lab -> 16 l/h to 130 l/h to 2000 l/h at production scale.
Easy to maintain, clean - CIP and SIP - and lower ongoing operating costs due to the high reliability of our technology.
HPH pretreatment can accelerate S. cerevisiae yeast autolysis which may result in higher protein, amino acid and total solids yield.
Production Scale
Lab Scale Cell Disruptor - modern 'French Press' type.
European CE and other international safety standards compliant construction.