A liposome is a spherical vesicle with a membrane composed of a phospholipid bilayer used to deliver drug or genetic material into a cell.
For STANSTED high pressure homogenisers to process liposomal formulations (depending upon the type of drug), 1000 bar to 2500 bar (14,500 psi to 36,000) and 1 or 2 passes are usually recommended. Particle size and PDI gets more reduced at higher pressures and increased number of passes.
Recently different liposomal trials were performed at varying pressure conditions to assess the efficiency of STANSTED high pressure homogenisers.
Liposomes loaded with hydrophilic and hydrophobic drugs were formulated and assessed for particle size and PDI. Both the size and PDIs were found to be significantly reduced at one pass.
Further size reductions and narrowing of PDI is possible; after increasing the pressure or performing multiple passes.
Liposomes - our High Pressure Homogenizers
Provide rapid reduction of vesicle size and lamellarity to achieve smaller sizes whilst narrowing the distribution.
HPH also presents the opportunity to load the liposomes, locating hydrophilic active substances within the aqueous vesicle core.
Ultra high pressures to 60,000 psi (4200 bar) and processing from 1 ml to 35ml in the Laboratory up to flow rates in excess of 1000 l/hour.
Easy to scale - from Lab to full production systems for continuous processing with repeatable results.
Multiple options for temperature control - both pre-processing and post processing.
Piston Gap and Micro-Channel Device (interaction chamber).
We think we are unique in that we offer interchangeable piston gap (PG) and micro-channel devices (MCD) processing heads for systems. For liposome preparation both types can be effective but we recommend (for production applications) that both technologies are tested.
Vesicle size reduction is a function of pressure, valve type (PG or MCD) and formulation. Typically sizes from micron level to below 50 nanometers / nm can be achieved.
Highly versatile, fully featured bench top instruments with pressures to 60,000 psi (410 MPa, 4200 bar).
For R&D and smaller scale production up to 125 l/hr.
Full scale production systems with flow rates up to 1000 l/hr offering unrivalled performance.